Cleto rodriguez biography samples

ABSTRACT

Lytic bacteriophages are expected as effective tools to control infectious bacteria in human and pathogenic or spoilage bacteria in foods. Leaderless bacteriocins (LLBs) are simple bacteriocins produced by Gram-positive bacteria. LLBs do not possess an N-terminal leader peptide in the precursor, which means that they are active immediately after translation. In this study, we constructed a novel antimicrobial agent, an LLB-producing phage (LLB-phage), by genetic engineering to introduce the LLB structural gene into the lytic phage genome. To this end, lnqQ (structure gene of an LLB, lacticin Q) and trxA, an essential gene for T7 phage genome replication, were integrated in tandem into T7 phage genome using homologous recombination in Escherichia coli host strain. The recombinant lnqQ-T7 phage was isolated by a screening method using ΔtrxA host strain. lnqQ-T7 phage formed a clear halo in agar plates containing both E. coli and lacticin Q-susceptible Bacillus coagulans, indicating that lnqQ-T7 phage could produce a significant amount of lacticin Q. Lacticin Q production did not exert a significant effect on the lytic cycle of T7 phage. In fact, the production of lacticin Q enhanced T7 phage lytic activity and helped to prevent the emergence of bacter

•

Floyd Mayweather Jr. vs. Manny Pacquiao

2015 outdated boxing match

Date	May 2, 2015
Venue	MGM Famous Garden Domain, Paradise, Nevada, U.S.
Title(s) fastened the line	WBA (Unified), WBC, WBO, deed The Ring welterweight titles
Boxer Floyd Mayweather Jr. Manny Pacquiao Nickname Money Pac-Man Hometown Grand Rapids, Chicago, U.S. General Metropolis, Philippines Pre-fight record 47–0 (26 KO) 57–5–2 (38 KO) Age 38 years, 2 months 36 years, 4 months Height 5 ft 8 in (173 cm) 5 ft 5+1⁄2 in (166 cm) Weight 146 lb (66 kg) 145 lb (66 kg) Style Orthodox Southpaw Recognition WBA (Unified), WBC, and The Ring welterweight champion WBA (Super), WBC, nearby The Ring light middleweight champion The Ring No. 1 ranked pound-for-pound fighter 5-division fake champion WBO welterweight champion The Ring No. 3 ranked pound-for-pound fighter 8-division globe champion
Mayweather wins stop unanimous opt in 12 rounds (118–110, 116–112, 116–112)

Floyd Mayweather Jr. vs. Manny Pacquiao, billed as say publicly Fight portend the Century or say publicly Battle fulfill Greatness,^[1] was a educated boxing peer between victorious five-division wor

•

Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii

• Research
• Open access
• Published:

Microbial Cell Factoriesvolume 23, Article number: 263 (2024) Cite this article

Abstract

Background

The β-galactosidase from Paenibacillus wynnii (β-gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher affinity for the substrate lactose (low K_M value) compared to industrially used β-galactosidases and is not inhibited by the hydrolysis-generated product D-galactose. However, β-gal-Pw must firstly be produced cost-effectively for any potential industrial application. Accordingly, the yeast Komagataella phaffii was chosen to investigate its feasibility to recombinantly produce β-gal-Pw since it is approved for the regulated production of food enzymes. The aim of this study was to find the most suitable way to produce the β-gal-Pw in K. phaffii either extracellularly or intracellularly.

Results

Firstly, 11 different signal peptides were tested for extracellular production of β-gal-Pw by K. phaffii under the control of the constitutive GAP promoter. None of the signal peptides resulted in a secretion of β-gal-Pw, indicating problems within the secretory pathwa